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SRX21480283: GSM7732842: Pg H2O2-1; Porphyromonas gingivalis W83; RNA-Seq
1 ILLUMINA (NextSeq 550) run: 12.6M spots, 959.6M bases, 365.1Mb downloads

External Id: GSM7732842_r1
Submitted by: Microbiology and Molecular Genetics, Loma Linda University
Study: Transcriptome analyses of Filifactor alocis ATCC35896 and Porphyromonas gingivalis W83 in mono-culture as compared to co-culture under hydrogen peroxide stress conditions
PRJNA1009061 • SRP456956 • All experiments • All runs
show Abstracthide Abstract
Oal anaerobic bacteria have to face constant oxidative stress in order to survive in the inflammatory environment of the periodontal pocket. In this study, we analyzed the transcriptome profiles of P. gingivalis and Filifactor in monoculture as compared to P. gingivalis+F.alocis coculture under H2O2 stress condtions, to analyze the genes responsible for enhanced survival of P. gingivalis in presence of F. alocis under oxidative-stress conditions. Overall design: RNA-seq was used to study the expression profiles of P. gingivalis as compared to P. gingivalis+F. alocis under stress conditions, and expression profile of F. alocis as compared to P. gingivalis+F. alocis under stress conditions respectively. Other experiments also designed and performed.
Sample: Pg H2O2-1
SAMN37145666 • SRS18712891 • All experiments • All runs
Organism: Porphyromonas gingivalis W83
Library:
Name: GSM7732842
Instrument: NextSeq 550
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Briefly, RNA were harvested by Qiagen RNA kit. ribosomal RNA was removed from 2 µg of total RNA by using RiboMinus Transcriptome Isolation Kit (Life Technologies, Carlsbad, CA). The first- and the second- strand cDNA synthesis, the RNA-seq barcode ligation, and other steps of libraries construction were performed by using Nextflex RNA-Seq kit according to the protocol of manufacturer (Bioo Scientific, Austin, TX). cDNA in library were taged by specific barcode sequence and exposed to sequencing.
Runs: 1 run, 12.6M spots, 959.6M bases, 365.1Mb
Run# of Spots# of BasesSizePublished
SRR2575730712,626,041959.6M365.1Mb2023-09-01

ID:
28921614

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